Molecular and cellular imaging

The molecular and cellular imaging core facility at RCSI primarily focuses on live cell imaging applications and supports users from different fields in biomedical research.

The facility employs confocal GFP imaging, fluorescence resonance energy transfer (FRET), fluorescence recovery after photo bleaching (FRAP), FLIP and photo-activation/photo-conversion techniques also in combination with patch clamp.

In cases of sophisticated applications, the facility gets involved in research projects, and further develops microscopic imaging applications to enhance the quality of the output of the state-of-the-art image acquisition setups based in RCSI.

With funding from the HEA, SFI, HRB, Cancer Research Ireland, and the EU the facility hosts:

  • Two epi-fluorescence microscopes with CCD camera
  • Three live cell epi-fluorescence imaging setups all suitable for fluorescence time lapse imaging under physiological conditions. One is additionally equipped with a microinjection system
  • Four confocal microscopes equipped for live cell imaging applications
  • One high content screening platform also equipped for live cell applications
  • IT Infrastructure for image storage, image processing and analysis

The facility also provides training in basic epi-fluorescence and confocal microscopy applications, image processing, and advanced live cell imaging.

For further information and requests please contact Dr Heiko Duessmann.